He spent the remaining 18 years of his life trying to decipher its mixture of plants, circles and humans praying to the fountain of youth but died before completing his task. The manuscript has been passed down through generations of scientists since then who have concluded that it is some kind of language – even if they don’t know what it means. The latest to take up the challenge were a team from the University of Arizona who dissected four 1mm by 6mm sections from four sample pages they were given by the Beinecke Rare Book and Manuscript Library of Yale University, which is where it is being stored. Other experts also looked at the paints and inks that were used by the mysterious author. Dr Greg Hodgins, an assistant research scientist and assistant professor in the UA’s department of physics, who led the project, said that they found some of the colours were consistent with those used in the Renaissance. But what really helped was the carbon dating process, which is where scientists measure the amount of radioisotope Carbon 14 which occurs naturally in objects and decays at a predictable rate, making it possible to use it to date things. That process allowed Dr Hodgins to pinpoint the early 15th century as the only time the manuscript could have been written.